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KMID : 0604520170430010019
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Journal of the Society of Cosmetic Scientists of Korea
2017 Volume.43 No. 1 p.19 ~ p.26
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Anti-inflammatory Effects of Amelanchier asiatica Fruits Ethanol Extract
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Lee Jin-Young
Yoo Dan-Hee
Joo Da-Hye
Kim So-Ra
Jo Hui-Seon
Joo Sung-Hyun
Chae Jung-Woo
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Abstract
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This study investigated the anti-inflammatory activities and cell viability of Amelanchier asiatica (A. asiatica) 70% ethanol extracts against RAW 264.7 cells induced by lipopolysaccharide (LPS). Cell toxicity test on macrophage cells (RAW 264.7) was performed by 3-[4,5-dimethyl-thiazol-2-yl]-2, 5-diphenyl-tetrazoliumbromide (MTT) assay and results showed 96% cell viability at 1,000 μg/mL concentration. Anti-inflammatory activity was examined via the inhibitory tests on the production of LPS induced NO in RAW 264.7 cells by Griess assay. The result showed that the extract inhibited NO production in concentration dependent manner. The iNOS and COX-2 protein expression inhibitory effects were confirmed by western blot and by reverse transcription-polymerase chain reaction (RT-PCR). From the former they were decreased by 84.3%, 56.2% at 500 μg/mL concentration, respectively, and from the latter decreased by 89.8%, 84.9% at 500 μg/mL, respectively. In conclusion, this study showed the anti-inflammatory effects of A. asiatica extracts. Thus, this could be applied to an anti-inflammatory agent.
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KEYWORD
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Amelanchier asiatica , iNOS , COX-2 , NO , anti-inflammatory
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